Cascadia Journal of Biomedical Research

Prognostic value of antimicrobial resistance at single-cell resolution

Rao, V., Engel, L.

Previous work on chronic kidney disease has produced conflicting results, partly because of differences in design and data. We find a 23% relative reduction in the primary outcome, and we show that the result is stable under a range of perturbations of the assumptions. Together, these results contribute to a more complete picture and open the way to further applications.

Corrigendum to “Improved catalytic efficiency of P450 OleP for converting lithocholic acid into murideoxycholic and ursodeoxycholic acids through semi-rational and rational design” [Synth Syst Biotechnol 15 (2027) 94-104]

Hu, B., Pang, C., Luo, D., Wang, Y., Sun, C., Zhou, J., Chen, J., Du, G., Zhong, F., Zhou, J., Wang, B., Zhao, X.

Combined magnetic resonance imaging and serum analysis reveals distinct multiple sclerosis types

Willard, C., Puglisi, L., Ravi, D., Dmitrieva, M., Mattiesing, R., Barkhof, F., Alexander, D., Harlow, D., Piani-Meier, D., Eshaghi, A.

Multiple sclerosis (MS) is a highly heterogeneous disease in its clinical manifestation and progression. Predicting individual disease courses is key for aligning treatments with underlying pathobiology. We developed an unsupervised machine learning model integrating MRI-derived measures with serum neurofilament light chain (sNfL) levels to identify biologically informed MS subtypes and stages. Us... [see more]

JAK-STAT pathway activation compromises nephrocyte function in a Drosophila high-fat diet model of chronic kidney disease

Zhao, Y., Duan, J., Seah, H., van de Leemput, J., Han, Z.

Chronic kidney disease is a major health issue and is gaining prevalence. Using a Drosophila model for chronic kidney disease, we show that a high-fat diet (HFD) disrupts the slit diaphragm filtration structure in nephrocytes, the fly functional equivalent of mammalian podocytes. The structural disruption resulted in reduced filtration function in the affected nephrocytes. We demonstrate that HFD ... [see more]

Improved catalytic efficiency of P450 OleP for converting lithocholic acid into murideoxycholic and ursodeoxycholic acids through semi-rational and rational design

Hu, B., Pang, C., Luo, D., Wang, Y., Sun, C., Zhou, J., Chen, J., Du, G., Zhong, F., Zhou, J., Wang, B., Zhao, X.

Cytochrome P450 enzymes are promising for synthesizing therapeutically beneficial bile acids murideoxycholic acid (MDCA) and ursodeoxycholic acid (UDCA) from lithocholic acid (LCA). However, the catalytic efficiency of P450 enzymes remains inadequate for large-scale production. Here, we address this limitation by engineering P450 OleP, achieving high activity for the efficient synthesis of MDCA an... [see more]

Post-traumatic stress status among emergency department nurses during the winter H1N1 influenza season and correlations with burnout, coping, resilience, and support (JOS DOCX counterpart)

Zheng, Z., Shen, D., Shi, Y., Chai, L., Zhou, Y.

Objective The H1N1 influenza, a highly contagious acute respiratory disease, causes a significant burden on public health, especially during the winter season when the number of cases surges. Emergency department nurses, who are on the frontline of patient care during this period, are exposed to high - stress situations, increasing their risk of developing post - traumatic stress disorder (PTSD).... [see more]

Baicalin regulates macrophages polarization and alleviates myocardial ischaemia/reperfusion injury via inhibiting JAK/STAT pathway

Xu, M., Li, X., Song, L.

Context Baicalin is an active compound which demonstrates cardioprotection effects against myocardial ischaemia/reperfusion injury (MI/RI). Objective To investigate how baicalin protects against myocardial injury and to explore its potential mechanism. We hypothesized that baicalin-modulated macrophages change from M1 (pro-inflammatory subset) to M2 (anti-inflammatory subset) under I/R stress. ... [see more]

Post-traumatic stress status among emergency department nurses during the winter H1N1 influenza season and correlations with burnout, coping, resilience, and support

Zheng, Z., Shen, D., Shi, Y., Chai, L., Zhou, Y.

Objective The H1N1 influenza, a highly contagious acute respiratory disease, causes a significant burden on public health, especially during the winter season when the number of cases surges. Emergency department nurses, who are on the frontline of patient care during this period, are exposed to high - stress situations, increasing their risk of developing post - traumatic stress disorder (PTSD).... [see more]

Correction to “Respiratory Support with Nasal High Flow Reduces Opioid Requirements During Endoscopic Retrograde Cholangiopancreatography”

A randomized trial of Parkinson’s disease across three population-based cohorts

Nguyễn, M., Okafor, T., Johansson, S.

Hypertension have attracted growing attention because of their relevance to both theory and practice. In contrast to earlier studies, our approach accounts for heterogeneity and avoids the usual simplifying assumptions. We find a distinct transcriptional program in the responding group. We discuss limitations of the present study and outline how the methods can be extended.